Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2β

被引：21
|
作者：
Burbelo, Peter D. [1 ]
Hirai, Hiroki [2 ]
Issa, Alexandra T. [1 ]
Kingman, Albert
Lernmark, Ake [3 ]
Ivarsson, Sten-A. [3 ]
Notkins, Abner L. [2 ]
Iadarola, Michael J. [1 ]
机构：
[1] Natl Inst Dent & Craniofacial Res, Neurobiol & Pain Therapeut Sect, Lab Sensory Biol, NIH, Bethesda, MD 20892 USA
[2] Natl Inst Dent & Craniofacial Res, Expt Med Sect, Oral Infect & Immun Branch, NIH, Bethesda, MD USA
[3] Malmo Univ Hosp, Dept Paediat, Malmo, Sweden
来源：
DIABETES CARE | 2010年 / 33卷 / 04期
基金：
美国国家卫生研究院;
关键词：
AUTOANTIGENS; DISEASE;
D O I：
10.2337/dc09-1938
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.
引用
收藏
页码：754 / 756
页数：3
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