Optimization of chitosan-alginate encapsulation process using pig Hepatocytes for development of bioartificial liver

被引：0
|
作者：
Lee, JH
Lee, DH
Son, JH
Park, JK
Kim, SK [1 ]
机构：
[1] Pukyong Natl Univ, Dept Biotechnol & Bioengn, Pusan 608737, South Korea
[2] Samsung Biomed Res Inst, Seoul 135710, South Korea
[3] Dongguk Univ, Dept Chem Engn, Seoul 100715, South Korea
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2005年 / 15卷 / 01期
关键词：
encapsulation; alginate; chitosan; collagen; hepatocyte; bioartificial liver;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Chitosan-alginate capsules were formed by electrostatic interactions and exhibited an appropriate mechanical strength, permeability, and stability for the culture of hepatocytes. Pig hepatocytes were isolated and hepatocyte spheroids formed and immobilized in chitosan-alginate capsules. An encapsulation procedure of 3 min and spheroid formation period of 24 h were the optimum conditions for the best liver functions. Pig hepatocytes with a cell density of 6.0 x 10(6) cells/ml in the capsules were found to be Most Suitable for application in a bioartificial liver Support system. The encapsulated pig hepatocyte spheroids exhibited stable ammonia removal and urea secretion rates in a bioreactor for 2 weeks. Accordingly, chitosan-alginate encapsulated hepatocyte spheroids in a packed-bed bioreactor would appear to have potential as a bioartificial liver.
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页码：7 / 13
页数：7
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