Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes

被引：11
|
作者：
Na, Kun
Choi, Seong-Jun
Kim, Sungwon
Sun, Bo Kyung
Woo, Dae Gyun
Chung, Hyung-Min
Park, Keun-Hong
机构：
[1] Pochon CHU Univ, Cell & Gene Therapy Res Inst, Coll Med, Seoul 135081, South Korea
[2] Catholic Univ, Div Biotechnol, Puchon 420743, South Korea
[3] Korea Inst Sci & Technol, Biomat Res Ctr, Seoul 136791, South Korea
[4] Chabiotech Co Ltd, Seoul 135081, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 10期
关键词：
ascorbate; cartilage; chondrocytes; dexamethasone; hydrogel;
D O I：
10.1007/s10529-007-9420-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.
引用
收藏
页码：1453 / 1457
页数：5
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  • [1] Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes
    Kun Na
    Seong-Jun Choi
    Sungwon Kim
    Bo Kyung Sun
    Dae Gyun Woo
    Hyung-Min Chung
    Keun-Hong Park
    [J]. Biotechnology Letters, 2007, 29 : 1453 - 1457
  • [2] Delivery of dexamethasone, ascorbate, and growth factor (TGF β-3) in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes
    Na, Kun
    Park, Ji Hyun
    Kim, Sung Won
    Sun, Bo Kyung
    Woo, Dae Gyun
    Chung, Hyung-Min
    Park, Keun-Hong
    [J]. BIOMATERIALS, 2006, 27 (35) : 5951 - 5957
  • [3] Combination of ascorbate and growth factor (TGF β-3) in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes for neocartilage formation
    Choi, Seong-Jun
    Na, Kun
    Kim, Sungwon
    Woo, Dae Gyun
    Sun, Bo Kyung
    Chung, Hyung-Min
    Park, Keun-Hong
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 83A (04) : 897 - 905
  • [4] Synergistic effect of TGFβ-3 on chondrogenic differentiation of rabbit chondrocytes in thermo-reversible hydrogel constructs blended with hyaluronic acid by in vivo test
    Na, Kun
    Kim, Sungwon
    Woo, Dae-Gyun
    Sun, Bo Kyung
    Yang, Han Na
    Chung, Hyung-Min
    Park, Keun-Hong
    [J]. JOURNAL OF BIOTECHNOLOGY, 2007, 128 (02) : 412 - 422
  • [5] Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2)
    Na, Kun
    Kim, Sung won
    Sun, Bo Kyung
    Woo, Dae Gyun
    Yang, Han Na
    Chung, Hyung Min
    Park, Keun Hong
    [J]. BIOMATERIALS, 2007, 28 (16) : 2631 - 2637
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