Use of CE for the determination of binding constants

被引：131
|
作者：
Jiang, Chunxia [1 ]
Armstrong, Daniel W. [1 ]
机构：
[1] Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA
来源：
ELECTROPHORESIS | 2010年 / 31卷 / 01期
关键词：
Affinity CE; Association constant; Binding constant; Frontal analysis; Hummel-Dreyer method; AFFINITY CAPILLARY-ELECTROPHORESIS; SERUM TRANSPORT PROTEINS; MONTE-CARLO-SIMULATION; ALA TERMINUS PEPTIDES; FRONTAL ANALYSIS; EQUILIBRIUM MIXTURES; ZONE-ELECTROPHORESIS; BETA-CYCLODEXTRIN; COMPUTER-SIMULATION; ASSOCIATION CONSTANTS;
D O I：
10.1002/elps.200900528
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In the past two decades, CE has been used frequently for the measurement of apparent binding/association constants. CE has numerous advantages, including short analysis times, low sample consumption, simplicity of operation, etc. This review provides a fundamental introduction to binding theory and then summarizes recent applications and advances in the field of CE-based methods for the evaluation of molecular association. The time period for this survey is from 2002 (when our previous review was published) to the present.
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页码：17 / 27
页数：11
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