High-Resolution Melting Analysis of BLU Methylation Levels in Gastric, Colorectal, and Pancreatic Cancers

被引：6

作者：

Shao, Yong ^{[1
,2
]}

Zhang, Wei ^{[3
,4
]}

Zhang, Chao ^{[2
]}

Wu, Qi ^{[2
]}

Yang, Hong ^{[2
,5
]}

Zhang, Jie ^{[1
]}

Guan, Ming ^{[6
]}

Wan, Jun ^{[2
,4
]}

Yu, Bo ^{[1
,2
]}

机构：

[1] Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen, Guangdong, Peoples R China

[2] Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China

[3] Ji Nan Univ, HKUST JNU Joint Lab, Guangzhou, Guangdong, Peoples R China

[4] Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Hong Kong, Peoples R China

[5] Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen, Guangdong, Peoples R China

[6] Fudan Univ, Dept Lab Med, Cent Lab, Huashan Hosp, Shanghai 200433, Peoples R China

来源：

CANCER INVESTIGATION | 2010年 / 28卷 / 06期

关键词：

BLU; Methylation; HRM; Gastric cancer; Colorectal cancer; Pancreatic cancer; SUPPRESSOR GENE BLU; DNA METHYLATION; PCR BIAS; 3P21.3; HYPERMETHYLATION; INACTIVATION; SENSITIVITY; RASSF1A; REGION;

D O I：

10.3109/07357901003631023

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BLU was recently characterized as a novel tumor suppressor gene (TSG), and was epigenetically silenced in some tumor cell lines and primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. We used HRM analysis to detect the methylation levels of BLU gene in 100 gastric, 100 colorectal, and 70 pancreatic cancers, and also in an equal number of adjacent normal tissues for all. The frequency of BLU methylation in all three types of cancer was significantly higher than that in normal tissues. And the expression levels of BLU were inversely correlated with methylation levels.

引用

页码：642 / 648

页数：7

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