Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy

被引：17
|
作者：
Shiga, H
Yamane, Y
Ito, E [1 ]
Abe, K
Kawabata, K
Haga, H
机构：
[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Kita Ku, Sapporo, Hokkaido 0600810, Japan
[2] Hokkaido Univ, Grad Sch Med, Dept Anat, Kita Ku, Sapporo, Hokkaido 0608638, Japan
[3] Hokkaido Univ, Grad Sch Sci, Div Phys, Kita Ku, Sapporo, Hokkaido 0600810, Japan
来源：
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS | 2000年 / 39卷 / 6B期
关键词：
actin filament; astrocyte; atomic force microscopy; cell membrane; elasticity; force mapping; Young's modulus;
D O I：
10.1143/JJAP.39.3711
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
In order to examine the mechanical properties of the membrane surface of astrocytes, we observed living astrocytes by atomic force microscopy (AFM) both in contact mode and force-mapping mode. Ridge-like structures reflecting actin filaments were observed in the topographic images in contact mode, but not in force-mapping mode, using a zero-loading force. When we measured the elasticity of astrocytes, we observed that the cell membrane above the nucleus was soft and the cell membrane above the cytosol was stiff. In particular, the parts reflecting actin filaments were very stiff. This effect of actin filaments on the elasticity of astrocytes was confirmed by the loss of actin filaments after application of actin-polymerization inhibitor.
引用
收藏
页码：3711 / 3716
页数：6
相关论文
共 50 条
  • [1] Surface structures of cultured type 2 astrocytes revealed by atomic force microscopy
    Yamane, Y
    Hatakeyama, D
    Haga, H
    Abe, K
    Ito, E
    [J]. ZOOLOGICAL SCIENCE, 1999, 16 (01) : 1 - 7
  • [2] Surface ultrastructure and mechanical propertyof human chondrocyte revealed by atomic force microscopy
    Hsieh, C. -H.
    Lin, Y. -H.
    Lin, S.
    Tsai-Wu, J. -J.
    Wu, C. H. Herbert
    Jiang, C. -C.
    [J]. OSTEOARTHRITIS AND CARTILAGE, 2008, 16 (04) : 480 - 488
  • [3] The use of atomic force microscopy to quantify membrane surface electrical properties
    Bowen, WR
    Doneva, TA
    Stoton, JAG
    [J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2002, 201 (1-3) : 73 - 83
  • [4] Surface architecture of Neisseria meningitidis capsule and outer membrane as revealed by atomic force microscopy
    Manno, Daniela Erminia
    Tala, Adelfia
    Calcagnile, Matteo
    Resta, Silvia Caterina
    Alifano, Pietro
    Serra, Antonio
    [J]. RESEARCH IN MICROBIOLOGY, 2021, 172 (06)
  • [5] Mechanical Properties of Various Phases on In/Si(111) Surfaces Revealed by Atomic Force Microscopy
    Iwata, Kota
    Yamazaki, Shiro
    Tani, Yuta
    Sugimoto, Yoshiaki
    [J]. APPLIED PHYSICS EXPRESS, 2013, 6 (05)
  • [6] Measurement of the mechanical properties of isolated tectorial membrane using atomic force microscopy
    Gueta, Rachel
    Barlam, David
    Shneck, Roni Z.
    Rousso, Itay
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (40) : 14790 - 14795
  • [7] Thermo-mechanical properties of the cell surface assessed by atomic force microscopy
    Starodubtseva, Maria N.
    Yegorenkov, Nikolai I.
    Nikitina, Irina A.
    [J]. MICRON, 2012, 43 (12) : 1232 - 1238
  • [8] Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy
    Lin, SM
    Lee, CK
    Lee, SY
    Kao, CL
    Lin, CW
    Wang, AB
    Hsu, SM
    Huang, LS
    [J]. CELLULAR MICROBIOLOGY, 2005, 7 (12) : 1763 - 1770
  • [9] Adsorption of oleate on fluorite surface as revealed by atomic force microscopy
    Chennakesavulu, K.
    Raju, G. Bhaskar
    Prabhakar, S.
    Nair, C. Madhavan
    Murthy, K. V. G. K.
    [J]. INTERNATIONAL JOURNAL OF MINERAL PROCESSING, 2009, 90 (1-4) : 101 - 104
  • [10] Macromolecular crystal structure and properties as revealed by atomic force microscopy
    McPherson, A
    [J]. METHODS IN MACROMOLECULAR CRYSTALLOGRAPHY, 2001, 325 : 1 - 8
12345