Impairment of myocardial angiogenic response in the absence of osteopontin

被引:23
|
作者
Zhao, Xue
Johnson, Jennifer N.
Singh, Krishna
Singh, Mahipal
机构
[1] E Tennessee State Univ, James H Quillen Coll Med, Dept Physiol, James H Quillen Vet Affairs Med Ctr, Johnson City, TN 37614 USA
[2] Second Mil Med Univ, Dept Cardiol, Changzheng Hosp, Shanghai, Peoples R China
关键词
angiogenesis; heart; myocardial infarction; osteopontin;
D O I
10.1080/10739680601139369
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Osteopontin (OPN), increased in the heart following myocardial infarction (MI), plays In important role in post-Ml remodeling. Angiogenesis, an important feature of tissue repair, begins in the infarcted myocardium within 3 days post-MI. Here, the authors studied the role of OPN in myocardial angiogenesis using wild-type (WT) and OPN knockout (KO) mice. Results: Measurement of angiogenic response using Griffonia simplicifolia lectin-1 (GSL-1) staining indicated reduced capillary density in the infarcted region of the OPN KO hearts as compared to WT hearts 7 and 14 days post-MI. Arteriolar density was lower in OPN KO hearts 14 days post-MI. The number of CD31 positive cells was also lower in the infarcted region of the OPN KO hearts as compared to WT hearts 14 days post-MI. In contrast, capillary and arteriolar densities in the noninfarcted regions of OPN KO and WT hearts were not significantly different. In vivo myocardial angiogenesis measured using Matrigel implantation in the left: ventricular myocardium indicated significant decrease in the percentage of vessel-like areas in the OPN KO vs. WT hearts. Furthermore. in vitro Matrigel lithe formation assay demonstrated a significant decrease in total tube length in cardiac microvascular endothelial cells (CMECs) isolated from OPN KO hearts as compared to CMECs front WT hearts. Treatment of OPN KO CMECs with purified OPN protein significantly increased total tube length, while bovine serum had no effect. Conclusion: Lack of OPN impairs myocardial angiogenic response, leading to adverse remodeling post-MI.
引用
收藏
页码:233 / 240
页数:8
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