Pseudomonas andropogonis is the causal agent of bacterial leaf stripe of sorghum (Sorghum bicolor (L.) Moench). Fifty grain sorghum accessions were evaluated for genetic variation to P. andropogonis over a 2-year period. Plants were inoculated at the 7 to 8 leaf stage of juvenile growth with an Idico filler-plug gun calibrated to deliver one mi of inoculum. Disease severity ratings were noted 2 weeks after inoculation on a 0 to 5 scale. For 1993 and 1994, disease severity ratings were significantly different (P < 0.01) among accessions and groups of resistant, intermediate, and susceptible germplasm. Significant differences (P < 0.05) were also observed between years. Resistant accessions included SC326-6, SC414-12E, BTX378, B35-6, and TX2862. Susceptible entries included TX2767, TX430, TAM428, BTx623, R9117, and 90B2662.