mFISH analysis reveals complexity of chromosome aberrations in individuals occupationally exposed to internal plutonium:: A pilot study to assess the relevance of complex aberrations as biomarkers of exposure to High-LET α particles

We recently demonstrated that a significant proportion of apparently stable insertions induced after exposure to a mean of one a particle/cell, detected using three-color FISH, were part of larger unstable complexes when visualized by 24-color FISH. Interestingly, regardless of the long-term persistence capability of the cell, the complexity of each alpha-particle-induced complex appeared to be specific to the nuclear traversal of a single a particle. To assess whether aberrations of a similar complexity are observed in vivo and also to examine the usefulness of detecting such aberrations as a biomarker of chronic exposure to a particles, we have carried out a limited pilot study of Russian workers with large body burdens of alpha-particle-emitting plutonium. We found unstable cells containing non-transmissible complex aberrations in all of the plutonium-exposed subjects analyzed by mFISH. In addition, all of the complexes seen were consistent with those previously observed in vitro. Non-transmissible complex aberrations were more common than transmissible-type complexes, consistent with ongoing/chronic exposure, and insertions were dominant features of both types of complex. Accordingly, this preliminary study supports the proposal that aberration complexity and non-transmissibility are the major cytogenetic features of a-particle exposure that could potentially be exploited as a specific indicator of chronic exposures to high-LET alpha particles. 2005 by Radiation Research Society.