HPTLC quantification of diosgenin in Dioscorea deltoidea: Evaluation of extraction efficacy, organ selection, drying method and seasonal variation

Dioscorea deltoidea (Dioscoreaceae) is an endangered species having tubers that possess anextensive range of therapeutic and economic value due to presence of bioactive compound diosgenin. Several industries have various necessities for compounds of specific classes and therefore, choosy extraction strategies for segregation of these classes ought to be produced.The current study aimed to screen potent part, identify best drying process, efficient extraction methods with solvent selection and seasonal variations on diosgenin content in D. deltoidea. The tuber contained the highest diosgenin content (1.15% DW) compared to leaf and stem. The highest yield of diosgenin was obtained using microwave-assisted extraction (MAE) with 50% ethanol (0.99% DW) and minimum incold maceration with water (0.46% DW). Shade drying was found to be the best drying method as compared to others in terms of diosgenin yield (1.43% DW) whereas sun drying produced low yield (1.12% DW). Estimation of yield and enriched diosgenin (1.19% DW) specified that winter (December) is the perfect time to harvest D. deltoidea.& nbsp; (c) 2021 SAAB. Published by Elsevier B.V. All rights reserved.