Effects of 2-deoxy-D-glucose on focal cerebral ischemia in hyperglycemic rats

被引:21
|
作者
Wei, JN
Cohen, DM
Quast, MJ
机构
[1] Univ Texas, Med Branch, Marine Biomed Inst, Dept Anat, Galveston, TX 77555 USA
[2] Univ Texas, Med Branch, Marine Biomed Inst, Dept Neurosci, Galveston, TX 77555 USA
[3] Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX 77030 USA
来源
关键词
cerebral ischemia; magnetic resonance imaging; NMR spectroscopy; hyperglycemia; 2-deoxy-D-glucose; lactate;
D O I
10.1097/01.WCB.0000056061.18772.72
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The authors examined the effects of pretreatment with 2-deoxy-D-glucose (2DG) on the middle cerebral artery occlusion-reperfusion (MCAO/R) model in hyperglycemic rats. Proton magnetic resonance imaging and spectroscopy were used to measure the lesion size, the level of cerebral perfusion deficit, and ratio of lactate to N-acetyl aspartate (NAA) in brain regions. By performing sequential diffusion weighted imaging, gradient echo bolus tracking, steady-state spin echo imaging, and water-suppressed proton magnetic resonance spectroscopy techniques, the time course of the early changes of the lactate/NAA peak ratio and perfusion deficit was examined in hyperglycemic rats undergoing 90-minute MCAO followed by 24-h reperfusion. Compared with the saline-treated hyperglycemic rats, 2DG treatment at 10 minutes before MCAO significantly reduced diffusion weighted imaging hyperintensity by similar to60% and the lactate/NAA peak ratio by similar to70% at 4 h after MCAO/R. Both spin echo-measured cerebral blood volume and dynamic gradient echo-relative cerebral blood flow showed that the restoration of blood supply recovered and remained at similar to80% of baseline during reperfusion in 2DG-treated hyperglycemic rats. These data suggest that inhibition of glucose metabolism by 2DG has a beneficial effect in reducing brain injury and minimizing the production of brain lactate during MCAO/R in hyperglycemic rats.
引用
收藏
页码:556 / 564
页数:9
相关论文
共 50 条
  • [1] Involvement of cerebral neurotensin in hyperglycemic response caused by 2-deoxy-D-glucose in rats
    Chun, SJ
    Niijima, A
    Shimizu, K
    Nagai, K
    [J]. NEUROSCIENCE LETTERS, 1995, 201 (03) : 191 - 194
  • [2] EFFECTS OF 2-DEOXY-D-GLUCOSE ON THERMOREGULATION IN RATS
    FORICHON, J
    MOTTAZ, M
    JOMAIN, MJ
    MINAIRE, Y
    [J]. JOURNAL DE PHYSIOLOGIE, 1974, 68 (03): : 351 - 358
  • [3] ENZYMIC AND CEREBRAL METABOLIC EFFECTS OF 2-DEOXY-D-GLUCOSE
    HORTON, RW
    MELDRUM, BS
    BACHELARD, HS
    [J]. JOURNAL OF NEUROCHEMISTRY, 1973, 21 (03) : 507 - 520
  • [4] TEMPORARY SUPPRESSION OF THE HYPERGLYCEMIC RESPONSE TO 2-DEOXY-D-GLUCOSE BY BLINDING
    YAMAMOTO, H
    NAGAI, K
    NAKAGAWA, H
    [J]. CHRONOBIOLOGY INTERNATIONAL, 1988, 5 (01) : 37 - 46
  • [5] CEREBRAL GLUCOSE-METABOLISM AND EEG EFFECTS OF 2-DEOXY-D-GLUCOSE IN PRIMATES
    HORTON, RW
    MELDRUM, BS
    [J]. ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY, 1973, 34 (01): : 109 - 109
  • [6] Effect of 2-deoxy-D-glucose on aminoacids metabolism in rats' cerebral cortex slices
    Muller, Alexandre P.
    Rotta, Liane N.
    Kawano, Cristina
    Leszczinski, Daniel N.
    Schweigert, Ingrid D.
    Londero, Lisiane G.
    Gravina, Fernanda S.
    da Silveira, Clarice K. B.
    de Souza, Carolina G.
    Battu, Cintia E.
    Goncalves, Carlos A.
    de Souza, Diogo O.
    Perry, Marcos L. S.
    [J]. NEUROCHEMICAL RESEARCH, 2006, 31 (03) : 417 - 422
  • [7] Effect of 2-deoxy-d-Glucose on Aminoacids Metabolism in Rats’ Cerebral Cortex Slices
    Alexandre P. Muller
    Liane N. Rotta
    Cristina Kawano
    Daniel N. Leszczinski
    Ingrid D. Schweigert
    Lisiane G. Londero
    Fernanda S. Gravina
    Clarice K.B. da Silveira
    Carolina G. de Souza
    Cíntia E. Battu
    Carlos A. Gonçalves
    Diogo O. de Souza
    Marcos L.S. Perry
    [J]. Neurochemical Research, 2006, 31 : 417 - 422
  • [8] CENTRAL SITE FOR HYPERGLYCEMIC ACTION OF 2-DEOXY-D-GLUCOSE IN MOUSE AND RAT
    MULLER, EE
    COCCHI, D
    FORNI, A
    [J]. LIFE SCIENCE PART 1 PHYSIOLOGY & PHARMACOLOGY, 1971, 10 (18): : 1057 - &
  • [9] CEREBRAL MICROVESSEL 2-DEOXY-D-GLUCOSE UPTAKE DURING ISCHEMIA-INDUCED SEIZURES
    NELL, JH
    WELCH, KMA
    [J]. ANNALS OF NEUROLOGY, 1980, 7 (05) : 457 - 461
  • [10] PERMISSIVE EFFECT OF VIP ON THE HYPERGLYCEMIC RESPONSE INDUCED BY 2-DEOXY-D-GLUCOSE
    SHIMIZU, K
    HIBINO, H
    KOMENAMI, N
    NAGAI, N
    NAGAI, K
    NAKAGAWA, H
    [J]. NEUROSCIENCE LETTERS, 1994, 175 (1-2) : 157 - 160