IgE binding synthetic peptides of Alt a 1, a major allergen of Alternaria alternata

被引：20

作者：

Kurup, VP ^{[1
]}

Vijay, HM

Kumar, V

Castillo, L

Elms, N

机构：

[1] Med Coll Wisconsin, Div Allergy Immunol, Milwaukee, WI 53226 USA

[2] Vet Adm Med Ctr, Res Serv 151 1, Milwaukee, WI 53295 USA

[3] Hlth Canada, Environm Hlth Directorate, Ottawa, ON K1A 0L2, Canada

来源：

PEPTIDES | 2003年 / 24卷 / 02期

关键词：

Alternaria allergen; B-cell epitope; IgE binding; asthma; synthetic peptides; ELISA;

D O I：

10.1016/S0196-9781(03)00024-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alternaria alternata protein, Alt a 1 is a major allergen associated with allergy in atopic patients. Although the molecule binds strongly to IgE antibody from patients, the epitopes involved have not been identified or defined. In the present study, we synthesized overlapping peptides spanning the whole sequence and evaluated their IgE binding with sera from patients with Alternaria-induced allergy. The results identified four IgE binding linear regions. Two of these regions K41-P50 and Y54-K63 showed consistent reactivity with all four patients studied. The specific epitopes involved in the immune response may be of value in the immunodiagnosis and probably also in specific immunotherapy. (C) 2003 Elsevier Science Inc. All rights reserved.

引用

页码：179 / 185

页数：7

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