Identification and characterization of the type 2C protein phosphatase Ptc4p in the human fungal pathogen Candida albicans

被引：8
|
作者：
Zhao, Jingwen [2 ]
Sun, Xin [2 ]
Fang, Jiaping [1 ]
Liu, Wei [2 ]
Feng, Chen [1 ]
Jiang, Linghuo [1 ,2 ]
机构：
[1] Tianjin Med Univ, Lab Mol Microbiol, Tianjian Res Ctr Basic Med Sci, Tianjin 300070, Peoples R China
[2] Tianjin Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
来源：
YEAST | 2010年 / 27卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Candida albicans; protein phosphatase; PTC4; mitochondrion; azole; SACCHAROMYCES-CEREVISIAE; SER/THR PHOSPHATASE; YEAST; PATHWAY; GENE; VIRULENCE; HOMOLOG; MORPHOGENESIS; INACTIVATION; INHERITANCE;
D O I：
10.1002/yea.1739
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ions. There are seven PP2C genes. named from PTC1 to PTC7. in Saccharomyces cerevrsiae. In the current study we identified the CaPTC4 gene in Candida albicans and demonstrated the CaPtc4p protein is a typical PP2C enzyme. which is highly conserved in fungal species. Deletion of CaPTC4 renders Candida cells sensitive: to sodium and potassium ions as well as for antifungal azole drugs. In addition. we have shown that CaPtc4p is localized in the mitochondrion. suggesting that CaPtc4p is likely to lie involved in the regulation of a mitochondrial function related too ion homeostasis. Copyright (C) 2009 John Wiley Sons. Ltd.
引用
收藏
页码：149 / 157
页数：9
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