Detection of pandemic influenza A/H1N1/2009 virus by real-time reverse transcription polymerase chain reaction

被引：7

作者：

Wu, Weili ^{[1
]}

Kang, Xiaoping ^{[2
,5
]}

Bai, Zhijun ^{[3
]}

Liu, Licheng ^{[1
]}

Li, Jingjing ^{[4
]}

Wu, Xinwei ^{[3
]}

Sun, Honghe ^{[1
]}

Hu, Tao ^{[1
]}

Yang, Min ^{[1
]}

Wang, Pengzhi ^{[1
]}

Liu, Li ^{[4
]}

Yang, Yinhui ^{[2
,5
]}

Di, Biao ^{[3
]}

Chen, Weijun ^{[1
,4
]}

机构：

[1] Chinese Acad Sci, Beijing Inst Genom, Beijing 101300, Peoples R China

[2] Acad Mil Med Sci, State Key Lab Pathogen & Biosecur, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China

[3] Ctr Dis Control & Prevent, Guangzhou 510080, Guangdong, Peoples R China

[4] Beijing BGI GBI Biotech Co Ltd, Beijing 101300, Peoples R China

[5] Acad Mil Med Sci, Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 165卷 / 02期

关键词：

Pandemic influenza A/H1N1/2009 virus; Real-time reverse transcription polymerase chain reaction (RT-PCR); Detection;

D O I：

10.1016/j.jviromet.2009.11.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The first case of pandemic influenza A/H1N1/2009 infection was reported in Mexico in mid-April, 2009, and to date the new H1N1 virus has spread to over 160 countries. Therefore, it is important to obtain reliable epidemiological data on the spread of this virus by novel molecular methods can be developed in detection of this new infectious agent. A new method was developed for detection of the pandemic influenza A/H1N1/2009 using real-time reverse transcription polymerase chain reaction (RT-PCR). Direct comparison with the specific primers and probes recommended by the World Health Organization (WHO) demonstrates that the new method has a much greater sensitivity, and may thus be employed as a rapid, alternative method in detecting of the pandemic influenza A/H1N1/2009 virus. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：294 / 296

页数：3

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