Development of polymerase chain reaction-based assays for bacterial gene detection

被引：43
|
作者：
Johnson, JR
机构：
[1] Vet Adm Med Ctr, Minneapolis, MN 55417 USA
[2] Univ Minnesota, Dept Med, Minneapolis, MN 55417 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2000年 / 41卷 / 03期
关键词：
polymerase chain reaction (PCR); primer design; review;
D O I：
10.1016/S0167-7012(00)00160-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Polymerase chain reaction-based assays provide rapid, simple, and sensitive detection of bacterial genes, but are not without their drawbacks. This review summarizes the principal advantages and disadvantages of PCR-based bacterial gene detection, provides guidelines for the development and validation of new PCR assays, and describes potential pitfalls that may be encountered and how these can be avoided. (C) 2000 Published by Elsevier Science B.V.
引用
收藏
页码：201 / 209
页数：9
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