Kinetics of transesterification catalyzed by immobilized lipase from Pseudomonas cepacia G63 in microaqueous system

The kinetics of transesterification for Sapium. Sebiferum oil with methanol catalyzed by immobilized lipase from Pseudomonas cepacia G63 in microaqueous system was investigated. Inhibition of methanol on lipase was observed. The reaction kinetics was successfully built according to "Ping-Pong Bi Bi" mechanism. The parameters in the proposed kinetic model were determined by experiment data. In consideration of transesterification reaction composed of three consecutive reversible steps, the reaction constants (k(1-6)) were calculated, and the results indicated that the second forward step (DG -> MG) was the limitation step for the reaction. The study provided a deep insight into understanding the enzymatic catalyzed transesterification of Sapium. Sebiferumoil, which will also be beneficial to the industrialization of this technology.