Site- and exercise-related variation in structure and function of cartilage from equine distal metacarpal condyle

被引:33
|
作者
Nugent, GE
Law, AW
Wong, EG
Temple, MM
Bae, WC
Chen, AC
Kawcak, CE
Sah, RL
机构
[1] Univ Calif San Diego, Dept Bioengn, San Diego, CA USA
[2] Univ Calif San Diego, Whitaker Inst Biomed Engn, San Diego, CA USA
[3] Colorado State Univ, Coll Vet Med & Biomed Sci, Orthopaed Res Lab, Dept Clin Sci, Ft Collins, CO USA
基金
美国国家航空航天局; 美国国家卫生研究院; 美国国家科学基金会;
关键词
equine; exercise training; articular cartilage; biomechanics;
D O I
10.1016/j.joca.2004.07.001
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Objective: Determine (1) the site-associated response of articular cartilage of the equine distal metacarpal condyle to training at a young age as assessed by changes in indentation stiffness and alterations in cartilage structure and composition, and (2) relationships between indentation stiffness and indices of cartilage structure and composition. Method. Experimental animals (n = 6) were trained on a track (increasing exercise to 1 km/day by 5 months); controls (n = 6) were pasture-reared. Animals were euthanized at 18 months and four osteochondral samples were harvested per metacarpal condyle from dorsal-medial, dorsal-lateral, palmar-medial, and palmar-lateral aspects. Cartilage was analyzed for India ink staining (quantified as reflectance score (RS)), short-term indentation stiffness (sphere-ended, 0.4 mm diameter), thickness, and biochemical composition. Results: Cartilage structural, biochemical and biomechanical properties varied markedly with site in the joint. Sites just medial and just lateral to the sagittal ridge showed signs of early degeneration, with relatively low RS, indentation stiffness, and collagen content, and relatively high water content. Effects of exercise and side (left vs right) were not detected for any measure. Overall, indentation stiffness correlated positively with RS and collagen content, and inversely with thickness and water content. Conclusion: Gentle exercise-imposed mechanical stimulation did not markedly affect articular cartilage function or structure. However, the marked site-associated variation suggests that biomechanical environment can initiate degenerative changes in immature cartilage during joint growth and maturation. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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页码:826 / 833
页数:8
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