Cloning and sequencing of the Aa-Pri1 gene specifically expressed during fruiting initiation in the edible mushroom Agrocybe aegerita, and analysis of the predicted amino-acid sequence

被引：45

作者：

Espinar, MTF ^{[1
]}

Labarère, J ^{[1
]}

机构：

[1] Univ Bordeaux 2, CRA Bordeaux, INRA, Lab Genet Mol & Ameliorat Champignons Cultives, F-33883 Villenave Dornon, France

来源：

CURRENT GENETICS | 1997年 / 32卷 / 06期

关键词：

Agrocybe aegerita; morphogenetic gene; fruiting; differentiation;

D O I：

10.1007/s002940050297

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A gene (Aa-Pri1) specifically expressed during fruiting initiation of the basidiomycete Agrocybe aegerita was cloned. The total length of the Aa-Pri1 gene was 492 bp including a class-II intron of 54 bp size located at nt +125; the open reading frame encoded for a 145-aa protein of 16093 Da. CCAAT (-156) and TATAAAT (-83) boxes, and T(A)(5)T(A)(2) (+593) and T(A)(3)T(A)(4)T(A)(6)T (+608) putative polyadenylation sequences were identified. The putative transcription start point was located at position 49. The Aa-Pri1 transcript was abundant only during fruiting initiation and was undetectable in the other stages of development. The Aa-Pri1 protein was hydrophilic, with a 20-aa hydrophobic motif in the NH2-terminal part, determining a putative alpha-helix. Two putative glycosylation sites were identified. Aa-Pri1 protein activity may be controlled by the phosphorylation of several residues by different protein kinases.

引用

页码：420 / 424

页数：5

共 7 条

[1] Cloning and sequencing of the Aa-Pri1 gene specifically expressed during fruiting initiation in the edible mushroom Agrocybe aegerita, and analysis of the predicted amino-acid sequence
Maria-Teresa Fernandez Espinar
J. Labarère
[J]. Current Genetics, 1997, 32 : 420 - 424
[2] Aa-Pri2, a single copy gene from Agrocybe aegerita, specifically expressed during fruiting initiation, encodes a hydrophobin with a leucine-zipper domain
Santos, C
Labarère, J
[J]. CURRENT GENETICS, 1999, 35 (05) : 564 - 570
[3] Aa-Pri2, a single-copy gene from Agrocybe aegerita, specifically expressed during fruiting initiation, encodes a hydrophobin with a leucine-zipper domain
Cruz Santos
J. Labarère
[J]. Current Genetics, 1999, 35 : 564 - 570
[4] Molecular characterization of the Pri3 gene encoding a cysteine-rich protein, specifically expressed during fruiting initiation within the Agrocybe aegerita complex
Sirand-Pugnet, P
Labarère, J
[J]. CURRENT GENETICS, 2002, 41 (01) : 31 - 42
[5] Molecular characterization of the Pri3 gene encoding a cysteine-rich protein, specifically expressed during fruiting initiation within the Agrocybe aegerita complex
Pascal Sirand-Pugnet
Jacques Labarère
[J]. Current Genetics, 2002, 41 : 31 - 42
[6] The Aa-Pri4 gene, specifically expressed during fruiting initiation in the Agrocybe aegerita complex, contains an unusual CT-rich leader intron within the 5′ uncoding region.
Pascal Sirand-Pugnet
Cruz Santos
Jacques Labarère
[J]. Current Genetics, 2003, 44 : 124 - 131
[7] The Aa-Pri4 gene, specifically expressed during fruiting initiation in the Agrocybe aegerita complex, contains an unusual CT-rich leader intron within the 5′ uncoding region.
Sirand-Pugnet, P
Santos, C
Labarère, J
[J]. CURRENT GENETICS, 2003, 44 (03) : 124 - 131

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