A novel xanthene-based colorimetric and fluorescence probe for detection of H2S in living cells

Hydrogen sulfide (H2S) is a signaling gasotransmitter that plays an important role in modulating the functions of different systems. We have reported a xanthene-based colorimetric and fluorescence Probe A for the selective detection of H2S over other sulfide-containing analytes. The emission intensity increases linearly with NaSH concentrations in the low concentration range suggested that it shows high sensitivity toward H2S with a detection limit as low as 7 nM. This probe exhibits an enhanced up to 250-fold fluorescence enhancement at. em 510 nm in the presence of H2S (50 mu M). In addition, Probe A can be applied for fluorescent imaging of H2S in living cells, providing a potentially powerful molecular imaging tool for imaging H2S.