A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Campylobacter jejuni, C-fetus, C-coli, C-upsaliensis, C-hyointestinalis, and C-lari

被引：10
|
作者：
Kamei, Kazumasa [1 ,2 ]
Kawabata, Hiroki [1 ,2 ]
Asakura, Masahiro [1 ]
Samosornsuk, Worada [1 ,3 ]
Hinenoya, Atsushi [1 ]
Nakagawa, Shinsaku [2 ]
Yamasaki, Shinji [1 ]
机构：
[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, 1-58 Rinku Ourai Kita, Izumisano, Osaka 5988531, Japan
[2] Osaka Univ, Grad Sch Pharmaceut Sci, Osaka, Japan
[3] Thammasat Univ, Fac Allied Hlth Sci, Bangkok, Thailand
来源：
JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2016年 / 69卷 / 03期
关键词：
CDT;
D O I：
10.7883/yoken.JJID.2015.182
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, we devised a multiplex PCR assay based on the gene of cytolethal distending toxin (cdt) B subunit to simultaneously detect and discriminate Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari. Species-specific PCR products were successfully obtained from all 38 C. jejuni, 12 C. fetus, 39 C. coli, 22 C. upsaliensis, 24 C. hyointestinalis, and 7 C. lari strains tested. On the other hand, no specific PCR products were obtained from other campylobacters and bacterial species tested (41 strains in total). The proposed multiplex PCR assay is a valuable tool for detection and descrimination of 6 major Campylobacter species, that are associated with gastrointestinal diseases in humans.
引用
收藏
页码：256 / 258
页数：3
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