Anti-tumor effect of carrimycin on oral squamous cell carcinoma cells in vitro and in vivo

被引:17
|
作者
Liang, Si-yuan [1 ]
Zhao, Tong-chao [1 ]
Zhou, Zhi-hang [1 ]
Ju, Wu-tong [1 ]
Liu, Ying [1 ]
Tan, Yi-ran [1 ]
Zhu, Dong-wang [1 ]
Zhong, Lai-ping [1 ]
机构
[1] Shanghai Jiao Tong Univ, Dept Oral & Maxillofacial Head & Neck Oncol, Shanghai Key Lab Stomatol,Peoples Hosp 9, Coll Stomatol,Sch Med,Natl Clin Res Ctr Oral Dis, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China
来源
TRANSLATIONAL ONCOLOGY | 2021年 / 14卷 / 06期
基金
中国国家自然科学基金;
关键词
Carrimycin; Antibiotics; Oral squamous cell carcinoma; PHASE-II; CANCER; EPOTHILONE; RAPAMYCIN; RECURRENT; PATHWAYS; TRIAL; HEAD;
D O I
10.1016/j.tranon.2021.101074
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: : Carrimycin is a newly synthesized macrolide antibiotic with good antibacterial effect. Exploratory experiments found its function in regulating cell physiology, proliferation and immunity, suggesting its potential anti-tumor capacity. The aim of this study is to investigate the anti-tumor effect of carrimycin against human oral squamous cell carcinoma cells in vitro and in vivo . Methods: : Human oral squamous cell carcinoma cells (HN30/HN6/Cal27/HB96 cell lines) were treated with gradient concentration of carrimycin. Cell proliferation, colony formation and migration ability were analyzed. Cell cycle and apoptosis were assessed by flow cytometry. The effect of carrimycin on OSCC in vivo was investigated in tumor xenograft models. Immunohistochemistry, western blot assay and TUNEL assays of tissue samples from xenografts were performed. The key proteins in PI3K/AKT/mTOR pathway and MAPK pathway were examined by western blot. Results: : As the concentration of carrimycin increased, the proliferation, colony formation and migration ability of OSCC cells were inhibited. After treating with carrimycin, cell cycle was arrested in G0/G1 phase and cell apoptosis was promoted. The tumor growth of xenografts was significantly suppressed. Furthermore, the expression of p-PI3K, p-AKT, p-mTOR, p-S6K, p-4EBP1, p-ERK and p-p38 were down-regulated in vitro and in vivo . Conclusions: : Carrimycin can inhibit the biological activities of OSCC cells in vitro and in vivo , and regulate the PI3K/AKT/mTOR and MAPK pathways.
引用
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页数:10
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