DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

被引：16

作者：

Alejandro Martinez-Roque, Mateo ^{[1
]}

Alberto Franco-Urquijo, Pablo ^{[1
]}

Miguel Garcia-Velasquez, Victor ^{[1
]}

Choukeife, Moujab ^{[2
]}

Mayer, Guenther ^{[2
]}

Molina-Ramirez, Sergio Roberto ^{[3
]}

Figueroa-Miranda, Gabriela ^{[3
]}

Mayer, Dirk ^{[3
]}

Alvarez-Salas, Luis M. ^{[1
]}

机构：

[1] IPN, Ctr Invest & Estudios Avanzados, Dept Genet & Biol Mol, Lab Terapia Gen, Cdmx 07360, Mexico

[2] Univ Bonn, Life & Med Sci LIMES Inst, D-53121 Bonn, Germany

[3] Forschungszentrum Julich, Inst Biol Informat Proc Bioelect IBI 3, D-52428 Julich, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2022年 / 645卷

关键词：

Aptamers; SELEX; SARS-CoV-2; COVID-19; Aptasensor; Capillary electrophoresis; IN-VITRO SELECTION; ELECTROPHORESIS-SELEX SELECTION; CAPILLARY-ELECTROPHORESIS; AFFINITY; CORONAVIRUS; EVOLUTION; DISCOVERY; PROTEINS; LIGANDS;

D O I：

10.1016/j.ab.2022.114633

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.

引用

页数：10

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