GENETIC ANALYSIS OF PLECTRANTHUS L. (LAMIACEAE) IN SAUDI ARABIA BASED ON RAPD AND ISSR MARKERS

被引:0
|
作者
Khalik, Kadry Abdel [1 ,2 ]
Osman, Gamal [1 ,3 ]
机构
[1] Umm Al Qura Univ, Biol Dept, Fac Sci, Mecca 673, Saudi Arabia
[2] Sohag Univ, Fac Sci, Bot Dept, Sohag 82524, Egypt
[3] Agr Genet Engn Res Inst AGERI Giza, Giza, Egypt
关键词
Plectranthus; Genetic diversity; ISSR; RAPD; Lamiaceae; Taxonomy; MEDICINAL-PLANTS; DIVERSITY; POPULATIONS;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The genetic diversity and phylogenetic analyses of seven species of the genus Plectranthus (Lamiaceae) from Saudi Arabia were carried out by using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Ten RAPD primers and five ISSR primers generated 137 polymorphic amplified fragments, which pointed a relatively high level of genetic variation in Plectranthus. RAPD markers revealed a higher level of polymorphism (105 bands) than ISSR (32 bands). The clustering of genotypes within groups showed difference upon comparison of RAPD and ISSR derived dendrograms. We could identify four clades within Plectranthus, which are largely in support, with a bit contradiction, of traditional groupings. Taxonomic and phylogenetic implications are discussed in comparison with the available gross morphological, anatomical, and phytochemical data. The results of this study present useful data for assessing the taxonomy of Plectranthus both at subgeneric and sectional levels. Moreover, our results indicate some level of resemblance among the species of subgenus Germanea and support the monophyly of this subgenus. The most interesting outcome of this analysis was identifying P. arabicus with distinguishing characters and suggesting that it should be treated as a distinct subgenus. In the same vein, distinguishing differences between the closely related endemic species P. asirensis and P. hijazensis were also noted suggesting that they should be placed in different subgenus. Similarly, P. asirensis and P. cylindraceus should be placed under a monophyletic group and this shows some closeness with P. tenuiflorus.
引用
收藏
页码:1073 / 1084
页数:12
相关论文
共 50 条
  • [1] Comparative Anatomy of Stems and Leaves of Plectranthus L. (Lamiaceae) in Saudi Arabia and Systematic Implications
    Khalik, Kadry N. Abdel
    Karakish, Eman A.
    [J]. MICROSCOPY RESEARCH AND TECHNIQUE, 2016, 79 (07) : 583 - 594
  • [2] A new species of Plectranthus (Lamiaceae) from Saudi Arabia
    Khalik, Kadry Abdel
    [J]. TURKISH JOURNAL OF BOTANY, 2016, 40 (05) : 506 - 513
  • [3] Genetic Diversity Analysis in the Genus Aloe vera (L.) Using RAPD and ISSR Markers
    Bhaludra, Chandra Sekhar Singh
    Yadla, Hari
    Cyprian, Farhan Sachal
    Bethapudi, Rama Rao
    Basha, S. D.
    Anupalli, Roja Rani
    [J]. INTERNATIONAL JOURNAL OF PHARMACOLOGY, 2014, 10 (08) : 479 - 486
  • [4] Investigation on molecular phylogeny of some date palm (Phoenix dactylifra L.) cultivars by protein, RAPD and ISSR markers in Saudi Arabia
    Abdulla, Munshi
    Gamal, Osman
    [J]. AUSTRALIAN JOURNAL OF CROP SCIENCE, 2010, 4 (01) : 23 - 28
  • [5] A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers
    ZHANG Guangqing QI Jianmin ZHANG Xiaochen FNAG Pingping SU Jianguang TAO Aifen LAN Tao WU Weiren and LIU Aimin Key Laboratory for Genetics Breeding and Multiple Utilization of Crops Ministry of Education Fuzhou PRChina Crop Sciences Institute Fujian Academy of Agricultural Sciences Fuzhou PRChina College of Life Science Fujian Agriculture and Forestry University Fuzhou PRChina Institute of Bast Fiber Crops Chinese Academy of Agricultural Sciences Changsha PRChina Department of Chemistry and Center for Metals in Biocatalysis University of Minnesota St Paul MN USA
    [J]. Agricultural Sciences in China., 2011, 10 (09) - 1353
  • [6] Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers
    Mir, Mudasir A.
    Mansoor, Sheikh
    Sugapriya, M.
    Alyemeni, Mohammed Nasser
    Wijaya, Leonard
    Ahmad, Parvaiz
    [J]. SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2021, 28 (02) : 1308 - 1317
  • [7] A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers
    Zhang Guang-qing
    Qi Jian-min
    Zhang Xiao-chen
    Fnag Ping-ping
    Su Jian-guang
    Tao Ai-fen
    Lan Tao
    Wu Wei-ren
    Liu Ai-min
    [J]. AGRICULTURAL SCIENCES IN CHINA, 2011, 10 (09): : 1346 - 1353
  • [8] Genetic diversity in a collection of Cucumis sativus L. assessed by RAPD and ISSR markers
    Shiragambi Hanumantagouda Manohar
    Hosakatte Niranjana Murthy
    K. V. Ravishankar
    [J]. Journal of Plant Biochemistry and Biotechnology, 2013, 22 : 241 - 244
  • [9] Genetic diversity in a collection of Cucumis sativus L. assessed by RAPD and ISSR markers
    Manohar, Shiragambi Hanumantagouda
    Murthy, Hosakatte Niranjana
    Ravishankar, K. V.
    [J]. JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 22 (02) : 241 - 244
  • [10] A comparative analysis of RAPD and ISSR markers for assessing genetic diversity in Iranian populations of Nigella sativa L.
    KorehKhosravi, Samira Hosseini
    Masoumiasl, Asad
    Dehdari, Masoud
    [J]. CELLULAR AND MOLECULAR BIOLOGY, 2018, 64 (01) : 52 - 59