Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis

被引:3
|
作者
Lee, Min-Ah [1 ]
Kang, Si-Mook [2 ]
Kim, Se-Yeon [1 ]
Kim, Ji-Soo [1 ]
Kim, Jin-Bom [1 ]
Jeong, Seung-Hwa [1 ]
机构
[1] Pusan Natl Univ, Sch Dent, Dept Prevent & Community Dent, PLUS Project BK21, Yangsan 50612, South Korea
[2] Yonsei Univ, Coll Dent, Dept Prevent Dent & Publ Oral Hlth, Seoul 03722, South Korea
基金
新加坡国家研究基金会;
关键词
Fusobacterium nucleatum; Porphyromonas gingivalis; QLF-D; red fluorescence; porphyrin; DENTAL MICROCOSM BIOFILM; RED AUTOFLUORESCENCE; AUTOINDUCER; BACTERIA; PLAQUE; COMMUNICATION; PORPHYRIN;
D O I
10.1007/s12275-018-7515-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.
引用
收藏
页码:628 / 633
页数:6
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