Formation of glycated recombinant leghemoglobin in Escherichia coli cells

被引：6
|
作者：
Kosmachevskaya, O. V. [1 ]
Topunov, A. F. [1 ]
机构：
[1] Russian Acad Sci, Bach Inst Biochem, Moscow 119071, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2010年 / 46卷 / 03期
基金：
俄罗斯基础研究基金会;
关键词：
ROOT-NODULES; PROTEIN; LIGAND; METHYLGLYOXAL; HEMOGLOBIN; REACTIVITY; NICOTINATE; PRODUCTS; ACIDS;
D O I：
10.1134/S0003683810030087
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A nonenzymatic glycation of the recombinant leghemoglobin expressed in Escherichia coli cells was demonstrated for the first time. This process involved the heme pocket and gave low-spin leghemoglobin species. A correlation between the degree of E. coli protein glycation and synthesis of poly-beta-hydroxybutyric acid was found, suggesting that the accumulation of reserve carbon sources and nonenzymatic glycation could be alternative processes.
引用
收藏
页码：297 / 302
页数：6
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