Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens

被引：21
|
作者：
Saikia, Deep Prakash [1 ]
Yadav, Kalpana [1 ]
Pathak, Dinesh C. [1 ]
Ramamurthy, Narayan [1 ]
D'Silva, Ajai Lawrence [1 ]
Marriappan, Asok Kumar [2 ]
Ramakrishnan, Saravanan [3 ]
Vakharia, Vikram N. [4 ]
Chellappa, Madhan Mohan [1 ]
Dey, Sohini [1 ]
机构：
[1] Indian Vet Res Inst, Div Vet Biotechnol, Recombinant DNA Lab, Bareilly 243122, Uttar Pradesh, India
[2] Indian Vet Res Inst, Div Pathol, Avian Dis Sect, Bareilly 243122, Uttar Pradesh, India
[3] Indian Vet Res Inst, Immunol Sect, Bareilly 243122, Uttar Pradesh, India
[4] Univ Maryland Baltimore Cty, Inst Marine & Environm Technol, Baltimore, MD 21202 USA
来源：
PATHOGENS | 2019年 / 8卷 / 03期
关键词：
newcastle disease virus; avian reovirus-sigma C protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy; INDUCED TENOSYNOVITIS; VACCINE VECTOR; STRAIN R2B; INFLUENZA; GENE; MAREKS; GENERATION; CHALLENGE; HERPESVIRUS; HENS;
D O I：
10.3390/pathogens8030145
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Newcastle disease (ND) and avian reovirus (ARV) infections are a serious threat to the poultry industry, which causes heavy economic losses. The mesogenic NDV strain R2B is commonly used as a booster vaccine in many Asian countries to control the disease. In this seminal work, a recombinant NDV strain R2B expressing the sigma C (sigma C) gene of ARV (rNDV-R2B-sigma C) was generated by reverse genetics, characterized in vitro and tested as a bivalent vaccine candidate in chickens. The recombinant rNDV-R2B-sigma C virus was attenuated as compared to the parent rNDV-R2B virus as revealed by standard pathogenicity assays. The generated vaccine candidate, rNDV-R2B-sigma C, could induce both humoral and cell mediated immune responses in birds and gave complete protection against virulent NDV and ARV challenges. Post-challenge virus shedding analysis revealed a drastic reduction in NDV shed, as compared to unvaccinated birds.
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页数：21
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