Preparation of aligned porous gelatin scaffolds by unidirectional freeze-drying method

被引：358
|
作者：
Wu, X. [1 ]
Liu, Y. [1 ]
Li, X. [2 ]
Wen, P. [1 ]
Zhang, Y. [2 ]
Long, Y. [1 ]
Wang, X. [1 ]
Guo, Y. [2 ]
Xing, F. [1 ]
Gao, J. [1 ]
机构：
[1] Tianjin Univ, Sch Sci, Tianjin 300072, Peoples R China
[2] Tianjin Orthoped Hosp, Tianjin 300211, Peoples R China
来源：
ACTA BIOMATERIALIA | 2010年 / 6卷 / 03期
基金：
美国国家科学基金会;
关键词：
Unidirectional freeze-drying; Gelatin; Scaffolds; Tissue engineering; CONTAINING ARTIFICIAL SKIN; GUIDANCE SCAFFOLDS; CELL AFFINITY; SPINAL-CORD; BONE; FIBERS; FABRICATION; ACID); SPONGES; LIQUID;
D O I：
10.1016/j.actbio.2009.08.041
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Porous gelatin scaffolds with microtubule orientation structure were manufactured by unidirectional freeze-drying technology, and their porous structure was characterized by scanning electron microscopy Scaffolds with tunable pore size and high porosity up to 98% were obtained by adjusting the concentration of the gelatin solution and crosslinking agent during the preparation process All the Porous gelatin scaffolds exhibited oriented microtubule pores, with width and length from 50 to 100 gm and 100 to 500 pm, respectively. Meanwhile, the properties of the scaffolds, such as porosity, water adsorption ability and compressive strength, were studied. In vitro enzymatic degradation results showed that the absolute weight loss of the gelatin scaffolds exhibited an increasing trend from low to high gelatin concentration used to prepare gelatin scaffolds, in vitro cell Culture results indicated that the porous gelatin scaffolds were non-toxic to cartilage cells, since the cells spread and grew well. (C) 2009 Acta Materialia Inc Published by Elsevier Ltd. All rights reserved
引用
收藏
页码：1167 / 1177
页数：11
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