The protein splicing domain of the homing endonuclease PI-SceI is responsible for specific DNA binding

被引：33
|
作者：
Grindl, W [1 ]
Wende, W [1 ]
Pingoud, V [1 ]
Pingoud, A [1 ]
机构：
[1] Univ Giessen, Inst Biochem, D-35392 Giessen, Germany
来源：
NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 08期
关键词：
D O I：
10.1093/nar/26.8.1857
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The homing endonuclease PI-SceI consists of a protein splicing domain (I) and an endonucleolytic domain (II). To characterize the two domains with respect to their contribution to DNA recognition we cloned, purified and characterized the isolated domains. Both domains have no detectable endonucleolytic activity. Domain I binds specifically to the PI-SceI recognition sequence, whereas domain II displays only weak non-specific DNA binding. In the specific complex with domain I the DNA is bent to a similar extent as observed with the initial complex formed between PI-SceI and DNA. Our results indicate that protein splicing domain I is also involved in recognition of the DNA substrate.
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收藏
页码：1857 / 1862
页数：6
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