Multiplex Immunofluorescence and Multispectral Imaging: Forming the Basis of a Clinical Test Platform for Immuno-Oncology

被引:33
|
作者
Hoyt, Clifford C. [1 ]
机构
[1] Akoya Biosci Inc, Malborough, MA USA
关键词
Predictive biomarkers; multiplex immunofluorescence; multispectral imaging; immuno-oncology; clinical workflow; image analysis; automated staining; spatial biology; LUNG-CANCER; EXPRESSION; ASSAY; IMMUNOHISTOCHEMISTRY; REPRODUCIBILITY; ASSOCIATION; NSCLC;
D O I
10.3389/fmolb.2021.674747
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As immuno-oncology (I/O) emerges as an effective approach in the fight against cancer, multispectral imaging of multiplex immunofluorescence (mIF) is maturing as an analytical platform. The timing is fortuitous. Due to health economic considerations surrounding the use of I/O, there is an urgent need for tests that accurately predict response to the growing list of available therapies. Multispectral mIF provides several advantages over other biomarker modalities by enabling deeper interrogation of the intricate biology within the tumor microenvironment, including detection of cell-to-cell spatial interactions that correlate with clinical outcomes. It also provides a practical path for generating reliable and reproducible results in a clinically suitable, high-throughput workflow. In this article, we (1) describe the principles behind multispectral mIF; (2) provide advice and recommendations on assay development and optimization and highlight characteristics of a well-performing assay; and (3) discuss the requirements for translating this approach into clinical practice.
引用
收藏
页数:14
相关论文
共 50 条
  • [1] Multiplex imaging in immuno-oncology
    Zhao, Chen
    Germain, Ronald N.
    JOURNAL FOR IMMUNOTHERAPY OF CANCER, 2023, 11 (10)
  • [2] Zmax test for delayed effect in immuno-oncology clinical trials
    Yang, Miao
    Hua, Zhaowei
    Xue, Lan
    Hu, Mingxiu
    JOURNAL OF BIOPHARMACEUTICAL STATISTICS, 2020, 30 (02) : 244 - 266
  • [3] Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
    Surace, Michael
    DaCosta, Karma
    Huntley, Anna
    Zhao, Weiguang
    Bagnall, Christopher
    Brown, Charles
    Wang, Chichung
    Roman, Kristin
    Cann, Jennifer
    Lewis, Arthur
    Steele, Keith
    Rebelatto, Marlon
    Parra, Edwin R.
    Hoyt, Clifford C.
    Rodriguez-Canales, Jaime
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2019, (143):
  • [4] Imaging the multiple facets of immuno-oncology
    Divgi, Chaitanya
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2018, 45 (01) : 121 - 122
  • [5] Imaging the multiple facets of immuno-oncology
    Chaitanya Divgi
    European Journal of Nuclear Medicine and Molecular Imaging, 2018, 45 : 121 - 122
  • [6] Clinical development of immuno-oncology therapeutics
    Wang, Jianxin
    Chen, Qi
    Shan, Qiang
    Liang, Tingbo
    Forde, Patrick
    Zheng, Lei
    CANCER LETTERS, 2025, 617
  • [7] The Clinical and Reimbursement Landscape of Immuno-Oncology
    Feinberg, Bruce A.
    Joseph, Richard W.
    Kolodziej, Michael
    AMERICAN JOURNAL OF MANAGED CARE, 2015, 21 : 3 - 4
  • [8] Clinical development of immuno-oncology in China
    Wu, Da-Wei
    Huang, Hui-Yao
    Tang, Yu
    Zhao, Yang
    Yang, Zhi-Min
    Wang, Jun
    Wang, Shu-Hang
    Yu, Yue
    Fang, Yuan
    Fang, Hong
    Bai, Ying
    Sun, Chao
    Fan, Qi
    Yu, An-Qi
    Wang, Huan-Ling
    Du, Chun-Xia
    Chen, Kun
    Huang, Ming-De
    Zhang, Yin
    Li, Ning
    Xu, Bing-He
    Sun, Yan
    He, Jie
    LANCET ONCOLOGY, 2020, 21 (08): : 1013 - 1016
  • [9] IMMUNOATLAS: AN ONLINE PUBLIC PORTAL FOR SHARING, VISUALIZING, AND REFERENCING MULTIPLEX IMMUNOHISTOCHEMISTRY/IMMUNOFLUORESCENCE (MIHC/IF) IMAGES AND RESULTS FOR IMMUNO-ONCOLOGY
    Lee, Jia Ying Joey
    Yeong, Joe
    Wen, Li
    Lee, Justina Nadia
    Loo, Lit-Hsin
    Dong, Jiahui
    JOURNAL FOR IMMUNOTHERAPY OF CANCER, 2021, 9 : A657 - A657
  • [10] MEASURES OF CLINICAL BENEFIT IN IMMUNO-ONCOLOGY STUDIES
    Zhao, L.
    Zhang, Y.
    Huang, Y.
    Huang, W.
    Mukhopadhyay, P.
    VALUE IN HEALTH, 2016, 19 (07) : A712 - A712