Solvent-free synthesis of glyceryl ferulate using a commercial microbial lipase

被引:50
|
作者
Sun, Shangde [1 ]
Shan, Liang [1 ]
Jin, Qingzhe [1 ]
Liu, Yuanfa [1 ]
Wang, Xingguo [1 ]
机构
[1] So Yangtze Univ, Key Lab Food Sci & Safety, Minist Educ, Sch Food Sci & Technol, Wuxi 214036, Jiangsu, Peoples R China
关键词
Candida antarctica lipase; ethyl ferulate; glyceryl ferulate; solvent-free synthesis; transesterification;
D O I
10.1007/s10529-007-9338-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A process was optimized for the enzymatic synthesis of glyceryl ferulate with a yield of up to 96% using a vacuum-rotary evaporation strategy under following conditions: 15 mmol glycerol, 1.5 mmol ethyl ferulate, 170 mg Candida antarctica lipase, at 60 degrees C for 10 h and under a vacuum of 10 mm Hg. The immobilized lipase can be used 10 times.
引用
收藏
页码:945 / 949
页数:5
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