Simple and High-Throughput Rheotaxis Behavioral Assay for Zebrafish Larva

被引：2
|
作者：
Tantry, M. S. Ananthakrishna [1 ]
Harini, V. S. [1 ]
Santhakumar, Kirankumar [1 ,2 ]
机构：
[1] SRM Inst Sci & Technol, Dept Genet Engn, Kattankulathur, India
[2] SRM Inst Sci & Technol, Dept Genet Engn, Kattankulathur 603203, India
来源：
ZEBRAFISH | 2022年 / 19卷 / 03期
关键词：
rheotaxis; zebrafish; behavior assay; copper sulfate; lateral line; ototoxicity; LATERAL-LINE; COPPER; FISH;
D O I：
10.1089/zeb.2021.0087
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Zebrafish (Danio rerio) is used as a model for studying sensorineural hearing loss. The damage to the hair cells can be assessed by scoring rheotaxis behavior in zebrafish. In this study, we newly designed a rheotaxis behavioral assay protocol capable of quantifying rheotaxis behavior in zebrafish larvae. We chemically induced ototoxicity in the larvae using copper sulfate, a well-known ototoxin, and determined rheotaxis at different flow velocities. The simple, cost-effective, and high-throughput rheotaxis assay system can provide great insights into drug development and other behavioral studies.
引用
收藏
页码：114 / 118
页数：5
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