Resveratrol ameliorates diet-induced dysregulation of lipid metabolism in zebrafish (Danio rerio)

Defective lipid metabolism is associated with increased risk of various chronic diseases, such as obesity, cardiovascular diseases, and diabetes. Resveratrol (RSV), a natural polyphenol, has been shown the potential of ameliorating disregulations of lipid metabolism. The objective of this study was to investigate the effects of feed intake and RSV on lipid metabolism in zebrafish (Danio rerio). The adult males were randomly allocated to 6 groups: control (Con, 8 mg cysts/fish/day), control with 20 ae mol/L RSV (Con+ RSV), calorie restriction (CR, 5 mg cysts/fish/day), calorie restriction with RSV (CR+ RSV), overfeed (OF, 60 mg cysts/fish/day), and overfeed with RSV (OF+ RSV) groups. The treatment period was 8 weeks. Results showed that CR reduced body length, body weight, and condition factor of zebrafish. CR reduced levels of plasma triglyceride (TG) and induced protein expression of phosphorylated AMP-activated protein kinase-a (pAMPK alpha), silent information regulator 2 homolog 1 (Sirt1), and peroxisome proliferator activated receptor gamma coactivator-1a (PGC1 alpha). RSV attenuated CR-induced pAMPKa/AMPKaincreases. RSV increased levels of Sirt1 protein in the OF zebrafish, and decreased OF-induced increase in peroxisome proliferator-activated receptor-gamma (PPAR gamma) protein level. Additionally, RSV down-regulated caveolin-1 and up-regulated microtubule-associated protein 1 light chain 3 -II (LC3-II) protein levels in OF zebrafish. In conclusion, these results suggest that 1) CR reduces plasma TG level through activation of the AMPK alpha-Sirt1-PGC1 alpha pathway; 2) under different dietary stress conditions RSV might regulate AMPK phosphorylation bi-directionally; 3) RSV might regulate lipid metabolism through the AMPK alpha-Sirt1-PPAR. pathway in OF zebrafish.