Laser speckle contrast imaging of cerebral blood flow of newborn mice at optical clearing

被引：2

作者：

Timoshina, Polina A. ^{[1
]}

Zinchenko, Ekaterina M. ^{[2
]}

Tuchina, Dania K. ^{[1
]}

Sagatova, Madina M. ^{[2
]}

Semyachkina-Glushkovskaya, Oxana V. ^{[2
]}

Tuchin, Valery V. ^{[1
,3
,4
]}

机构：

[1] Saratov Natl Res State Univ, Res Educ Inst Opt & Biophoton, Astrakhanskaya 83, Saratov 410012, Russia

[2] Saratov Natl Res State Univ, Dept Biol, Astrakhanskaya 83, Saratov 410012, Russia

[3] Inst Precis Mech & Control RAS, Lab Laser Diagnost Tech & Living Syst, Rabochaya 24, Saratov 410028, Russia

[4] Natl Res Tomsk State Univ, Interdisciplinary Lab Biophoton, Lenins Av 36, Tomsk 634050, Russia

来源：

SARATOV FALL MEETING 2016 OPTICAL TECHNOLOGIES IN BIOPHYSICS AND MEDICINE XVIII | 2017年 / 10336卷

基金：

俄罗斯科学基金会;

关键词：

cerebral blood flow; laser speckle contrast imaging; newborn; mice; skin; fontanelle; optical clearing; glycerol; Omnipaque (TM);

D O I：

10.1117/12.2268358

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work, we consider the use of optical clearing agents to improve imaging quality of the cerebral blood flow of newborn mice. Aqueous 60%-glycerol solution, aqueous 70%-Omnipaque (TM)(300) solution and Omnipaque (TM) (300) solution in water/DMSO(25%/5%) were selected as the optical clearing agents. Laser speckle contrast imaging (LSCI) was used for imaging of cerebral blood flow in newborn mice brain during topical optical clearing of tissuesin the area of the fontanelle. These results demonstrate the effectiveness of glycerol and Omnipaque solutions as optical clearing agents for investigation of cerebral blood flow in newborn mice without scalp removing and skull thinning.

引用

页数：7

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