Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

被引：15
|
作者：
Culebras, Mario [1 ,2 ]
Grande, Cristian J. [3 ]
Torres, Fernando G. [3 ]
Troncoso, Omar P. [3 ]
Gomez, Clara M. [1 ,2 ]
Carmen Bano, M. [2 ,4 ]
机构：
[1] Univ Valencia, Dept Quim Fis, E-46100 Burjassot, Spain
[2] Univ Valencia, Inst Ciencia Mat, E-46100 Burjassot, Spain
[3] Pontificia Univ Catolica Peru, Dept Mech Engn, Lima 32, Peru
[4] Univ Valencia, Fac Biol, Dept Bioquim Biol Mol 1, E-46100 Burjassot, Spain
来源：
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS | 2015年 / 64卷 / 08期
关键词：
Bacterial cellulose; cell growth; poly-L-lysine; collagen; tissue engeneering; MICROBIAL CELLULOSE; MORPHOLOGICAL CHARACTERIZATION; POTENTIAL SCAFFOLD; IN-VIVO; ADHESION; ACID; BIOCOMPATIBILITY; COMPOSITE; SUBSTRATE; MEMBRANES;
D O I：
10.1080/00914037.2014.958829
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates. [GRAPHICS] .
引用
收藏
页码：411 / 415
页数：5
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