Identification of eicosanoids in the red algae, Gracilaria asiatica, using high-performance liquid chromatography and electrospray ionization mass spectrometry

被引：38
|
作者：
Sajiki, J
Kakimi, H
机构：
[1] Publ Hlth Lab Chiba Prefecture, Chuo Ku, Chiba 260, Japan
[2] JASCO Corp, Liquid Chromatog Div, Hachioji, Tokyo 192, Japan
来源：
JOURNAL OF CHROMATOGRAPHY A | 1998年 / 795卷 / 02期
关键词：
Gracilaria asiatica; eicosanoids; leukotrienes; hydroxytetraenoic acids; prostaglandins;
D O I：
10.1016/S0021-9673(97)00943-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Identification of eicosanoids which are metabolites of arachidonic acid in red algae Gracilaria asiatica, one of the popular seaweeds in Japan, was carried out using high-performance liquid chromatography (HPLC) interfaced with electrospray ionization mass spectrometry. Prostaglandin (PG) E-2, 19-keto-PGE(2), and 8-hydroxyeicosatetraenoic acid (HETE) were detected as major eicosanoids and PGA(2), leukotriene B-4 as minor ones in G. asiatica. 8- and 12-HETE had the same retention time in HPLC analysis, but using this analytical method, we were able to identify them. (C) 1998 Elsevier Science B.V.
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页码：227 / 237
页数：11
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