Surprising Results from a Search for Effective Disinfectants for Tobacco mosaic virus-Contaminated Tools

Over 100 years after its discovery, Tobacco mosaic virus (TMV) remains an economically important pathogen for producers of many vegetatively propagated crops including petunias (Petunia x hybrida) To directly address this concern, we have developed a robust system to determine efficacy of disinfectants for treating TMV-contaminated cutting tools using a combination of preliminary screens and replicated trials Contrary to widely held beliefs, wild-type (wt) TMV and four additional tobamovirus species infected four petunia cultivars without producing obvious viral symptoms In contrast, a petunia isolate of TMV with 99 0% (nucleotide) and 99 4% (amino acid) coat protein sequence identity to wt TMV produced symptoms on all but one tested cultivar We also show that TMV transmission can occur up to the twentieth petunia plant cut following a single cutting event on a TMV-infected plant Although many new products are now available, treatment of TMV-contaminated tools with a 20% (wt/vol) solution of nonfat dry milk (NFDM) plus 0 1% Tween 20 or a 1 10 dilution of household bleach (0 6% sodium hypochlorite), two "old standbys". completely eliminated TMV transmission to petunias Treatment of contaminated tools with 1% (wt/vol) Vitkon S or 20% NFDM also significantly reduced the incidence of infected petunias Other treatments identified in the preliminary screens are candidates for the second phase of screening that simulates contamination during the process of taking cuttings