Peptide nucleic acid-mediated competitive PCR clamping for detection of rifampin-resistant Mycobacterium tuberculosis

被引：8

作者：

Iwamoto, T ^{[1
]}

Sonobe, T ^{[1
]}

机构：

[1] Kobe Inst Hlth, Dept Microbiol, Chuo Ku, Kobe, Hyogo 6500046, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 10期

关键词：

D O I：

10.1128/AAC.48.10.4023-4026.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Peptide nucleic acid-mediated competitive PCR clamping, which can selectively amplify mutant alleles, was developed to detect mutations in four codons (513, 516, 526, and 531) of the rpoB gene in Mycobacterium tuberculosis strains. This simple method successfully identified the mutations in all 40 of the M. tuberculosis strains tested.

引用

页码：4023 / 4026

页数：4

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