Replication Attempt: "Effect of BMAP-28 Antimicrobial Peptides on Leishmania Major Promastigote and Amastigote Growth: Role of Leishmanolysin in Parasite Survival''

被引：3

作者：

Iorns, Elizabeth ^{[1
]}

Gunn, William ^{[1
]}

Erath, Jessey ^{[2
]}

Rodriguez, Ana ^{[2
]}

Zhou, Jian ^{[3
]}

Benzinou, Michael ^{[1
]}

机构：

[1] Reproducibil Initiat, Palo Alto, CA 94301 USA

[2] NYU, Sch Med, Dept Microbiol, Antiinfect Screening Core Facil, New York, NY 10016 USA

[3] LifeTein LLC, Hillsborough, NJ USA

来源：

PLOS ONE | 2014年 / 9卷 / 12期

关键词：

D O I：

10.1371/journal.pone.0114614

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study describes an attempt to replicate experiments from the paper "Effect of BMAP-28 Antimicrobial Peptides on Leishmania major Promastigote and Amastigote Growth: Role of Leishmanolysin in Parasite Survival,'' which was submitted to the Reproducibility Initiative for independent validation. The cathelicidin bovine myeloid antimicrobial peptide 28 (BMAP-28) and its isomers were previously shown to have potent antiparasitic activity against Leishmania major. We tested the effectiveness of L-BMAP-28 and two of its isomers, the D-amino acid form (D-BMAP-28) and the retro-inverso form (RI-BMAP-28), in both unamidated and amidated forms, as anti-leishmanial agents against Leishmania major promastigotes in vitro. We observed that L-BMAP-28, as well as its D and RI isomers, demonstrate anti-leishmanial activity against L. major promastigotes in vitro. The inhibitory effect was lower than what was seen in the original study. At 2 mu M of amidated peptides, the viability was 94%, 36%, and 66% with L-, D-and RIpeptides, versus 57%, 6%, and 18% in the original study.

引用

页数：13