Note: Dynamic point spread function for single and multiphoton fluorescence microscopy

被引：5
|
作者：
Mondal, Partha Pratim [1 ]
Mandal, Subhra [2 ]
Diaspro, Alberto [3 ]
机构：
[1] Indian Inst Sci, Dept Instrumentat, Bangalore 560012, Karnataka, India
[2] SISSA, I-34149 Trieste, Italy
[3] Italian Inst Technol, I-16163 Genoa, Italy
来源：
REVIEW OF SCIENTIFIC INSTRUMENTS | 2010年 / 81卷 / 04期
关键词：
bioluminescence; biomedical optical imaging; fluorescence; molecular biophysics; optical microscopy; optical transfer function; ENDOPLASMIC-RETICULUM; 2-PHOTON EXCITATION; FIELD; DIFFRACTION; RESOLUTION; TRAFFICKING; IMPROVEMENT; RECEPTOR; LIMIT;
D O I：
10.1063/1.3378683
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
We propose and demonstrate a dynamic point spread function (PSF) for single and multiphoton fluorescence microscopy. The goal is to generate a PSF whose shape and size can be maneuvered from highly localized to elongated one, thereby allowing shallow-to-depth excitation capability during active imaging. The PSF is obtained by utilizing specially designed spatial filter and dynamically altering the filter parameters. We predict potential applications in nanobioimaging and fluorescence microscopy.
引用
收藏
页数：3
相关论文
共 50 条
  • [1] Multispot point spread function for multiphoton fluorescence microscopy
    Mondal, Partha Pratim
    REVIEW OF SCIENTIFIC INSTRUMENTS, 2009, 80 (09):
  • [2] Point Spread Function Engineering for Super-Resolution Single-Photon and Multiphoton Fluorescence Microscopy
    Mondal, Partha Pratim
    Diaspro, Alberto
    ADVANCES IN IMAGING AND ELECTRON PHYSICS, VOL 175, 2013, 175 : 201 - +
  • [3] In-depth point-spread-function characterization in multiphoton fluorescence microscopy
    Lee, YC
    Dong, CY
    So, P
    BIOPHYSICAL JOURNAL, 2002, 82 (01) : 494A - 494A
  • [4] POINT-SPREAD FUNCTION RETRIEVAL FOR FLUORESCENCE MICROSCOPY
    Pankajakshan, Praveen
    Blanc-Feraud, Laure
    Kam, Zvi
    Zerubia, Josiane
    2009 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1 AND 2, 2009, : 1095 - +
  • [5] Single shot, three dimensional fluorescence microscopy with a spatially rotating point spread function
    Wang, Zhaojun
    Cai, Yanan
    Liang, Yansheng
    Zhou, Xing
    Yan, Shaohui
    Dan, Dan
    Bianco, Piero R.
    Lei, Ming
    Yao, Baoli
    BIOMEDICAL OPTICS EXPRESS, 2017, 8 (12): : 5493 - 5506
  • [6] Measurement and restoration of the point spread function of fluorescence confocal microscopy
    Yoo, H
    Song, I
    Gweon, DG
    JOURNAL OF MICROSCOPY, 2006, 221 (172-176) : 172 - 176
  • [7] Point spread function engineering with multiphoton SPIFI
    Wernsing, Keith A.
    Field, Jeffrey J.
    Domingue, Scott R.
    Allende-Motz, Alyssa M.
    DeLuca, Keith F.
    Levi, Dean H.
    DeLuca, Jennifer G.
    Young, Michael D.
    Squier, Jeff A.
    Bartels, Randy A.
    THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXIII, 2016, 9713
  • [8] 3D Single-Molecule Super-Resolution Fluorescence Microscopy with the Corkscrew Point Spread Function
    Lee, Maurice
    Lew, Matthew
    von Diezmann, Alex
    Weiss, Lucien
    Shechtman, Yoav
    Moerner, W. E.
    BIOPHYSICAL JOURNAL, 2016, 110 (03) : 176A - 176A
  • [9] Multiphoton fluorescence microscopy
    Gratton, E
    Barry, NP
    Beretta, S
    Celli, A
    METHODS, 2001, 25 (01) : 103 - 110
  • [10] Fast and accurate three-dimensional point spread function computation for fluorescence microscopy
    Li, Jizhou
    Xue, Feng
    Blu, Thierry
    JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2017, 34 (06) : 1029 - 1034
12345