COMPARISON OF RESAZURIN AND MTT METHODS ON STUDIES OF CITOTOXICITY IN HUMAN TUMOR CELL LINES

被引：0
|
作者：
Escobar M, Linamaria [1 ]
Rivera, Augusto [2 ]
Aristizabal G, Fabio A. [1 ]
机构：
[1] Univ Nacl Colombia, Fac Ciencias, Dept Farm, Grp Farmacogenet Canc, Bogota 14490, Colombia
[2] Univ Nacl Colombia, Fac Ciencias, Dept Quim, Bogota 14490, Colombia
来源：
VITAE-REVISTA DE LA FACULTAD DE QUIMICA FARMACEUTICA | 2010年 / 17卷 / 01期
关键词：
Doxorubicin; MTT; resazurine; cellular viability; ALAMAR-BLUE; IN-VITRO; CYTOTOXICITY TESTS; MICROTITER ASSAY; GROWTH; PROLIFERATION; MITOCHONDRIAL; VIABILITY; CANCER;
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学科分类号：
摘要：
A study was carried comparing the resazurina and MTT methods as indicators of indirect measure of cellular survival applied to valuated cytotoxic activity in order to assess the suitability of each of the two detection systems. The comparison was made through the valuation of cytotoxicity on three cell lines derived from human tumors: HEp-2, HT-29 and HeLa, evaluating three synthetic compounds during a treatment period of 48 hours using as a reference activity Doxorubicin HCl. The results obtained by the two methods showed a similar performance under the conditions tested in cell lines.
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页码：67 / 74
页数：8
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