FT-IR spectral features of DNA as markers for the detection of liver preservation using irradiation

被引:13
|
作者
Hamad, Amany M. [1 ]
Fahmy, Heba M. [1 ]
Elshemey, Wael M. [1 ,2 ]
机构
[1] Cairo Univ, Fac Sci, Biophys Dept, Giza 12613, Egypt
[2] Islamic Univ, Fac Sci, Dept Phys, Madinah, Saudi Arabia
关键词
FT-IR spectroscopy; Calf liver; DNA damage; Sterilization; Irradiated food; Gamma irradiation; CALF-THYMUS DNA; TRANSFORM INFRARED-SPECTROSCOPY; ESCHERICHIA-COLI O157H7; GENOMIC DNA; UV DEGRADATION; FOOD; DAMAGE; ACID; TEMPERATURE; SENSITIVITY;
D O I
10.1016/j.radphyschem.2019.108522
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Food preservation, inhibition of foodborne-pathogenic microorganisms, as well as prolonging the shelf life of food products are all achieved through the application of irradiation technology in food preservation. In terms of food safety and for strengthening worldwide trade of irradiated foods, the presence of a trustworthy method for identification of irradiated foodstuff is currently the focus of attention. Since DNA has a documented high sensitivity to ionizing radiation, this work introduces a method for the detection of gamma-irradiated (3 kGy) calf liver through measurements of Fourier-transform infrared (FT-IR) spectra of their extracted DNA. Results show characteristic FT-IR bands that yield significant differences between irradiated and control calf liver samples. The guanine nucleoside band at 1491 cm(-1) provides the most significant characterization.
引用
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页数:6
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