Immunostick colorimetric assay for detection of Pseudomonas spp. in refrigerated meat and milk

被引：3

作者：

Gutierrez, R ^{[1
]}

Garcia, T ^{[1
]}

Gonzalez, I ^{[1
]}

Sanz, B ^{[1
]}

Hernandez, PE ^{[1
]}

Martin, R ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT NUTR & BROMATOL 3,MADRID 28040,SPAIN

来源：

JOURNAL OF FOOD PROTECTION | 1997年 / 60卷 / 08期

关键词：

immunostick colorimetric assay; immunostick ELISA; refrigerated meat; refrigerated milk; Pseudomonas fluorescens; Pseudomonas assay;

D O I：

10.4315/0362-028X-60.8.908

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an immunostick enzyme-linked immunosorbent assay (ELISA) format for the detection of Pseudomonas spp. in refrigerated meat and milk. The detection threshold for the immunostick ELISA assay developed in this work is 10(4) CFU cm(-2) for meat and 10(5) CRT ml(-1) for milk samples.

引用

页码：908 / 911

页数：4

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