Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation

被引：23

作者：

Wang, H. H. ^{[1
,2
,3
]}

Cui, Q. ^{[3
]}

Zhang, T. ^{[2
,3
]}

Wang, Z. B. ^{[2
]}

Ouyang, Y. C. ^{[2
]}

Shen, W. ^{[3
]}

Ma, J. Y. ^{[3
]}

Schatten, H. ^{[4
]}

Sun, Q. Y. ^{[1
,2
,3
]}

机构：

[1] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China

[2] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China

[3] Qingdao Agr Univ, Shandong Univ, Coll Anim Sci & Technol, Inst Reprod Sci,Key Lab Anim Reprod & Germplasm E, Qingdao 266109, Peoples R China

[4] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2016年 / 145卷 / 06期

关键词：

Meiotic maturation; Cortical granules; Rab3A; Rab27A; Rab35; CORTICAL GRANULES; MAMMALIAN EGGS; CAENORHABDITIS-ELEGANS; ASYMMETRIC DIVISION; SECRETORY GRANULES; PORCINE OOCYTE; CELL-DIVISION; IN-VITRO; ACTIN; FERTILIZATION;

D O I：

10.1007/s00418-015-1404-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation. The three Rab family members showed different localization patterns in oocytes. Microinjection of siRNA, antibody injection, or inhibitor treatment showed that (1) Rab3A regulates peripheral spindle and cortical granule (CG) migration, polarity establishment, and asymmetric division; (2) Rab27A regulates CG exocytosis following MII-stage oocyte activation; and (3) Rab35 plays an important role in spindle organization and morphology maintenance, and thus meiotic nuclear maturation. These results show that Rab proteins play important roles in mouse oocyte meiotic maturation and activation and that different members exert different distinct functions.

引用

页码：647 / 657

页数：11

共 20 条

[1] Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation
H. H. Wang
Q. Cui
T. Zhang
Z. B. Wang
Y. C. Ouyang
W. Shen
J. Y. Ma
H. Schatten
Q. Y. Sun
Histochemistry and Cell Biology, 2016, 145 : 647 - 657
[2] Differential dynamics of Rab3A and Rab27A on secretory granules
Handley, Mark T. W.
Haynes, Lee P.
Burgoyne, Robert D.
JOURNAL OF CELL SCIENCE, 2007, 120 (06) : 973 - 984
[3] Rab3A and Rab27A cooperatively regulate the docking of dense-core vesicle
Tsuboi, Takashi
Fukuda, Mitsunori
NEUROSCIENCE RESEARCH, 2006, 55 : S68 - S68
[4] Rab27A and Rab27B Regulate Secretion in Mouse Pancreatic Acini through Different Pathways
Hou, Y.
Lentz, S. I.
Williams, J. A.
PANCREAS, 2014, 43 (08) : 1367 - 1367
[5] Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes
Martinez-Arroyo, Olga
Flores-Chova, Ana
Sanchez-Garcia, Belen
Redon, Josep
Cortes, Raquel
Ortega, Ana
BIOLOGY-BASEL, 2023, 12 (05):
[6] Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells
Tsuboi, Takashi
Fukuda, Mitsunori
JOURNAL OF CELL SCIENCE, 2006, 119 (11) : 2196 - 2203
[7] Grab recruitment by Rab27A-Rabphilin3a triggers Rab3A activation in human sperm exocytosis
Florencia Quevedo, Maria
Alberto Bustos, Matias
Masone, Diego
Roggero, Carlos Marcelo
Martin Bustos, Diego
Nora Tomes, Claudia
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2019, 1866 (04): : 612 - 622
[8] Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2
Matsunaga, Kohichi
Taoka, Masato
Isobe, Toshiaki
Izumi, Tetsuro
JOURNAL OF CELL SCIENCE, 2017, 130 (03) : 541 - 550
[9] Rab3D and Rab27B Cooperatively Regulate Secretion in Mouse Pancreatic Acini
Hou, Y.
Chen, X.
Williams, J. A.
PANCREAS, 2013, 42 (08) : 1355 - 1355
[10] NONNEURONAL EXPRESSION OF RAB3A - INDUCTION DURING ADIPOGENESIS AND ASSOCIATION WITH DIFFERENT INTRACELLULAR MEMBRANES THAN RAB3D
BALDINI, G
SCHERER, PE
LODISH, HF
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) : 4284 - 4288

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