Immunohistochemical study of renal fibropoiesis associated with dogs naturally and experimentally infected with two different strains of Leishmania (L.) infantum

被引：8
|
作者：
Alves, Adriano F. [1 ]
Pereira, Ramon A. [1 ]
de Andrade, Helida M. [2 ]
Mosser, David M. [3 ]
Tafuri, Wagner L. [1 ]
机构：
[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol, Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, Brazil
[3] Univ Maryland, Maryland Pathogen Res Inst, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA
来源：
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 2019年 / 100卷 / 04期
关键词：
BH401; strain; canine visceral leishmaniasis; fibrosis; kidney; CANINE VISCERAL LEISHMANIASIS; INTRALOBULAR LIVER FIBROSIS; MESENCHYMAL STEM-CELLS; ACUTE KIDNEY INJURY; FOLLOW-UP; MOLECULAR-MECHANISMS; EXPERIMENTAL-MODEL; MEDIATED-IMMUNITY; KALA-AZAR; NEPHROPATHY;
D O I：
10.1111/iep.12321
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The objectives of this work were to study some pathological aspects of kidneys obtained from dogs naturally infected with Leishmania infantum and from dogs experimentally infected with two different strains of L infantum with special emphasis on fibrotic process. Seventy eight specimens of paraffin-embedded kidney fragments were collected as follows: (a) CNI group composed by 62 kidney samples of adult mongrel dogs, naturally infected with L infantum; (b) BH401 group composed by five kidney samples of adult Beagles experimentally infected with L infantum strain MCAN BR/2002/BH401; (c) BH400 group composed by eleven kidney samples of adult Beagles experimentally infected with L infantum strain MCAN/BR/2000/BH400, at the same dose and same route of the previous group, denominated group BH400; Control group (CC) composed by four kidney samples of adult Beagles. All animals revealed glomerular and interstitial fibropoiesis associated with different types of glomerulonephritis and chronic interstitial nephritis. Fibrosis was markedly more intense in the BH401 group, followed by animals in the CNI group. Markers for myofibroblasts (mesenchymal markers) such as alpha-actin (alpha-SMA), vimentin and the cytokine transforming growth factor beta (TGF-beta) were done by immunohistochemistry. BH401 group showed higher expression of all these markers than others. Intracellular amastigotes forms of Leishmania was mainly found in BH401. These results could be indicating that the MCAN/BR/2002/BH401 strain is a good choice for the study of renal LVC experimental model.
引用
收藏
页码：222 / 233
页数：12
相关论文
共 50 条
  • [1] RENAL LESIONS IN DOGS NATURALLY INFECTED WITH Leishmania infantum
    Wilson, Tais Meziara
    Magalhaes, Larissa Fernandes
    Souza, Rafael Rocha
    Medeiros-Ronchi, Alessandra Aparecida
    Limongi, Jean Ezequiel
    BIOSCIENCE JOURNAL, 2017, 33 (04): : 990 - 995
  • [2] Immune response against Leishmania antigens in dogs naturally and experimentally infected with Leishmania infantum
    Rhalem, A
    Sahibi, H
    Guessous-Idrissi, N
    Lasri, S
    Natami, A
    Riyad, M
    Berrag, B
    VETERINARY PARASITOLOGY, 1999, 81 (03) : 173 - 184
  • [3] Hepatic fibropoiesis in dogs naturally infected with Leishmania (Leishmania) infantum treated with liposome-encapsulated meglumine antimoniate and allopurinol
    Castro, R. S.
    de Amorim, I. F. G.
    Pereira, R. A.
    Silva, S. M.
    Pinheiro, L. J.
    Pinto, A. J. W.
    Azevedo, E. G.
    Demicheli, C.
    Caliari, M. M. V.
    Mosser, D. M.
    Michalick, M. S. M.
    Frezard, Frederic J. G.
    Tafuri, Wagner L.
    VETERINARY PARASITOLOGY, 2018, 250 : 22 - 29
  • [4] Biomarkers and renal arterial resistive index in dogs naturally infected with Leishmania infantum
    Fabiano de Oliveira Frazilio
    Fernando de Almeida Borges
    Alda Izabel de Souza
    Mariana Isa Poci Palumbo
    Carlos Alberto do Nascimento Ramos
    Diogo Helney Freire
    André Luiz Baptista Galvão
    Mariana Green de Freitas
    Fernanda Battistotti Barbosa
    Parasitology Research, 2018, 117 : 3399 - 3405
  • [5] Biomarkers and renal arterial resistive index in dogs naturally infected with Leishmania infantum
    Frazilio, Fabiano de Oliveira
    Borges, Fernando de Almeida
    de Souza, Alda Izabel
    Poci Palumbo, Mariana Isa
    do Nascimento Ramos, Carlos Alberto
    Freire, Diogo Helney
    Baptista Galvao, Andre Luiz
    de Freitas, Mariana Green
    Barbosa, Fernanda Battistotti
    PARASITOLOGY RESEARCH, 2018, 117 (11) : 3399 - 3405
  • [6] Asymptomatic colitis in naturally infected dogs with Leishmania infantum:: A prospective study
    Adamama-Moraitou, Katerina K.
    Rallis, Timoleon S.
    Koytinas, Alexander F.
    Tontis, Dimitris
    Plevraki, Katerina
    Kritsepi, Maria
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 76 (01): : 53 - 57
  • [7] CELLULAR AND HUMORAL IMMUNE-RESPONSES IN DOGS EXPERIMENTALLY AND NATURALLY INFECTED WITH LEISHMANIA-INFANTUM
    PINELLI, E
    KILLICKKENDRICK, R
    WAGENAAR, J
    BERNADINA, W
    DELREAL, G
    RUITENBERG, J
    INFECTION AND IMMUNITY, 1994, 62 (01) : 229 - 235
  • [8] Seizures as a Consequence of Hyperviscosity Syndrome in Two Dogs Naturally Infected with Leishmania infantum
    Proverbio, Daniela
    Spada, Eva
    Perego, Roberta
    de Giorgi, Giada Bagnagatti
    JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION, 2016, 52 (02) : 119 - 123
  • [9] Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum
    Aldair JW Pinto
    Maria M Figueiredo
    Fabiana L Silva
    Trycia Martins
    Marilene SM Michalick
    Washington L Tafuri
    Wagner L Tafuri
    Acta Veterinaria Scandinavica, 53
  • [10] Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum
    Pinto, Aldair J. W.
    Figueiredo, Maria M.
    Silva, Fabiana L.
    Martins, Trycia
    Michalick, Marilene S. M.
    Tafuri, Washington L.
    Tafuri, Wagner L.
    ACTA VETERINARIA SCANDINAVICA, 2011, 53
12345