Preparation and characterization of sericin powder extracted from silk industry wastewater

In this study, we developed a new effective technology for the extraction of sericin from silk wastewater. Sericin was extracted with 75% (v/v) ethanol to obtain crude powder. The chemical composition of sericin powder, including protein, sugar, ash, and amino acid, was assayed in detail. The molecular weight distribution of sericin was also investigated by gel filtration chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and high performance liquid chromatography (HPLC) analytical methods. The results suggested that sericin represented a family of proteins with wide-ranging molecular weight distribution. The conformation changed in the course of ethanol precipitation was studied by circular dichroism (CD) analysis. Data implied that the major conformation of sericin protein was random coil, which decreased slightly after being treated with ethanol, and the appearance of beta-Turn conformation maybe associated with the packing of molecular chains induced by ethanol. Furthermore, sericin was found to inhibit tyrosinase activity when chlorogenic acid was used as a substrate, and had obvious radical scavenging effects with the 2.2-diphenyl-1-picryl-hydrazil (DPPH) assay. Result suggested that sericin might be a valuable ingredient for food. (c) 2006 Elsevier Ltd. All rights reserved.