Advances in cryo-electron tomography and subtomogram averaging and classification

被引：58
|
作者：
Zhang, Peijun [1 ,2 ,3 ]
机构：
[1] Univ Oxford, Div Struct Biol, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England
[2] Diamond Light Source, Electron Bioimaging Ctr, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England
[3] Univ Pittsburgh, Sch Med, Dept Biol Struct, Pittsburgh, PA 15260 USA
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2019年 / 58卷
基金：
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词：
IN-VIVO STRUCTURES; MOLECULAR ARCHITECTURE; CHEMORECEPTOR ARRAYS; 3D RECONSTRUCTION; SITU STRUCTURE; REVEALS; MICROSCOPY; VISUALIZATION; PROTEASOMES; RESOLUTION;
D O I：
10.1016/j.sbi.2019.05.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cryo-electron tomography (cryoET) can provide 3D reconstructions, or tomograms, of pleomorphic objects such as organelles or cells in their close-to-native states. Subtomograms that contain repetitive structures can be further extracted and subjected to averaging and classification to improve resolution, and this process has become an emerging structural biology method referred to as cryoET subtomogram averaging and classification (cryoSTAC). Recent technical advances in cryoSTAC have had a profound impact on many fields in biology. Here, I review recent exciting work on several macromolecular assemblies demonstrating the power of cryoSTAC for in situ structure analysis and discuss challenges and future directions.
引用
收藏
页码：249 / 258
页数：10
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