A Frequency Domain SIM Reconstruction Algorithm Using Reduced Number of Images

被引:38
|
作者
Lal, Amit [1 ,2 ]
Shan, Chunyan [3 ]
Zhao, Kun [1 ]
Liu, Wenhui [4 ]
Huang, Xiaoshuai [5 ]
Zong, Weijian [5 ]
Chen, Liangyi [5 ]
Xi, Peng [1 ,2 ]
机构
[1] Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
[2] Shanghai Univ Sci & Technol, Sch Optelect & Comp Engn, Shanghai 200093, Peoples R China
[3] Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China
[4] Tsinghua Univ, Dept Automat, Beijing 100084, Peoples R China
[5] Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
基金
中国国家自然科学基金;
关键词
Structured illumination; SIM; super-resolution; optical transfer function; ordinary least square (OLS); STRUCTURED-ILLUMINATION MICROSCOPY; FLUORESCENCE MICROSCOPY; EXCITATION MICROSCOPY; RESOLUTION; SUPERRESOLUTION; PHASE; OPTIMIZATION; SPECTRA;
D O I
10.1109/TIP.2018.2842149
中图分类号
TP18 [人工智能理论];
学科分类号
081104 ; 0812 ; 0835 ; 1405 ;
摘要
Conventional 2D structured illumination microscopy (SIM) requires nine raw images to reconstruct a super-resolved image. In order to increase the frame rate of 2D SIM, attempts are being made to reduce the number of raw SIM images. However, all the proposed SIM reconstruction algorithms (SIM-RA) capable of reconstructing super-resolution (SR) image with a reduced number of raw SIM images operate in the spatial domain. Here, we present a frequency domain SIM-RA based on ordinary least squares technique, which enables reconstruction of SR image using four raw SIM images. Unlike the spatial domain RA, which produces the SR image through iterative convergence, the presented RA provides a single-step solution. It also reveals the fundamental limitation of least number of raw images required for resolution doubling in SIM.
引用
收藏
页码:4555 / 4570
页数:16
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