Antioxidant effect of erdosteine and lipoic acid in ovarian ischemia-reperfusion injury

Objective: To investigate the effects of erdosteine and alpha lipoic acid (ALA) in a rat model of ovarian ischaemia-reperfusion injury. Study design: Forty-eight female Wistar albino rats were separated, at random, into six groups of eight rats. The groups were classified as: sham, torsion, detorsion, detorsion + erdosteine 100 mg/kg, detorsion + alpha lipoic acid (ALA) 100 mg/kg, and detorsion + erdosteine + ALA. The investigators executing the biochemical and histological analyses were blinded to the randomization until the end of the study. Results: The TOS (Total Oxidant Status) and OSI (Oxidative Stress Index) levels are higher in the Torsion and Detorsion groups when compared with the ones in the Sham group (p < 0.05). Strong correlation was found between OSI and total histological score in the sham, torsion and detorsion groups (r = 0.765, p < 0.001). The mean levels of TOS and OSI in the rats that received erdosteine and/or ALA were significantly lower compared with the sham, torsion and detorsion groups (p < 0.05). Mean TOS and mean OSI were lower in the detorsion + erdosteine + ALA group compared with the detorsion + erdosteine and detorsion + ALA groups (p < 0.05). In comparison with the detorsion group, the numbers of primordial follicles (p = 0.006) and primary follicles (p = 0.036) were increased in the groups that received erdosteine and/or ALA. Conclusions: Erdosteine and ALA decreased ischaemia-reperfusion injury in an experimental rat ovarian torsion model; combination treatment had a greater effect than either agent alone. Treatment with erdosteine and/or ALA was found to preserve the loss of reproductive capacity normally observed after ovarian torsion. (C) 2014 Elsevier Ireland Ltd. All rights reserved.