Encapsulation of purified lactoferrin from camel milk on calcium alginate nanoparticles and its effect on growth of osteoblasts Cell Line MG-63

In the present study, the lactoferrin (LF) that had been extracted from camel milk (through ion-exchange chromatography) has been loaded onto hydrogel particles of calcium alginate (Alg), while its effects on osteoblast cells line MG63 have been thoroughly evaluated. The extracted LF has been purified by the application of HPLC and SDS-PAGE techniques. Subsequent to being exposed to Alg (two concentrations of 0.2% and 0.5% in three pHs 6, 7, and 8), the interactions between LF and Alg have been studied by multiple spectroscopic as well as the zeta potential, and DLS particle size distribution. Fluorescence spectroscopy results have displayed the quenched emissions of proteins upon their interactions with Alg. In accordance with the obtained results, the zeta potential value of LF has faced a decrease through the formation of LF-Alg complex. Moreover, an outcome of 89.9% of loading has been perceived in both concentrations of Alg that the utilization of SEM electron microscopy. Once the loading had been confirmed, the effect of the particles that have been loaded on the MG63 cell line was put under investigation through the employment of MTT test. Taken together, it has been indicated by the achieved results that LF and Alg alone cannot cause any toxicity on the MG63 cells, while significantly increase their growth by being added to the cell culture media. The results have suggested that throughout all of the samples with evident increased cell growth, the highest interaction between LF and Alg has been observed to be at pH 8.