A prospective comparison between stabilized glutaraldehyde and chlorhexidine gluconate for Preoperative skin antisepsis in dogs

Objective-To compare the efficacy of 0.3% stabilized glutaraldehyde and alcohol (SG + A), 0.3% SG and water (SG + W), and 4% chlorhexidine gluconate tincture (CG + A), as skin disinfectants in dogs undergoing ovariohysterectomy. Study Design-Prospective, blinded clinical study. Animals-One hundred and twenty-one dogs. Methods-Cutaneous bacterial colony forming units (CFU) from the perioperative site after skin preparation, after antisepsis, and after surgery (incisional and paramedian), were quantified. The influence of high initial bacterial counts (greater than or equal to 150 CFU) and surgical time on antibacterial efficacy was examined and the proportion of dogs from which Staphylocoecus intermedius was cultured, determined. Perioperative skin reactions and wound infections were documented. Results-All 3 antiseptic solutions significantly and equally reduced CFU to all post-antisepsis sampling levels irrespective of surgical duration (mean surgical times 151.6, 136.2, and 149.6 minutes for CG + A, SG + A and SG + W, respectively). Median percentage reductions in CFU ranged between 99.3% and 100%. In dogs with initial high counts and disinfected with CG+A and SG + W, the incisional samples had significantly higher counts than the post-antisepsis samples. In the CG + A and SG + W groups, the proportion of post-surgery samples yielding S. intermedius was significantly higher at the incisional than the paramedian sites. Eight mild cutaneous reactions were recorded in equal proportions for the 3 solutions. There were no recorded infections. Conclusions-All 3 preparations had an equal ability to reduce and maintain low CFU counts, with minimal cutaneous reactions. Clinical Relevance-SG solutions are safe and effective preoperative skin antiseptics for elective clean-contaminated surgical procedures. (C) Copyright 2004 by The American College of Veterinary Surgeons.